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Separation of [3H]estradiol-17β binding proteins by electrofocusing in flat beds of Sephadex

✍ Scribed by A.I. Coffer; R.J.B. King

Publisher: Elsevier Science
Year: 1976
Tongue: English
Weight: 731 KB
Volume: 72
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(76)90519-4

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✦ Synopsis

The development of a technique is described for rapid analytical or preparative electrofocusing of uterine estrogen-binding proteins in flat beds of Sephadex gel. The method has been used to obtain data for different forms of rat uterine estrogen-binding proteins. I5e technique enables a distinction to be made between different tissue and plasma estrogen-bound components. An added advantage is that only components with klgh affinity for steroid are electrofocused.

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