Separation by high-performance liquid chromatography of penicillins with C4 to C10 aliphatic side chains

✍ Scribed by JoséM. Luengo; Miguel A. Moreno

• Publisher: Elsevier Science
• Year: 1987
• Tongue: English
• Weight: 310 KB
• Volume: 164
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(87)90533-1

✦ Synopsis

A suitable and rapid method for the simultaneous determination of different aliphatic penicillins is described. Butyryl-, pentanoyl-, hexanoyl-, heptanoyl-, octanoyl-, nonanoyl-and decanoylpenicillin can be completely separated by high-performance liquid chromatography using an isocratic elution mode (50 mM H,KPO,, pH 50methano1, 45:55 v/v). Under these conditions, retention times for C4 to Cl0 aliphatic side-chain penicillins were 2.5, 2.8, 4.1, 5.8, 8.9, 15.3, and 28.2 min. The benzylpenicillin retention time was 3.3 min.