Separation and spectrophotometric determination of theophylline and hydroxyethyltheophylline in a pharmaceutical syrup

✍ Scribed by Fabrizio De Fabrizio

• Publisher: John Wiley and Sons
• Year: 1978
• Tongue: English
• Weight: 224 KB
• Volume: 67
• Category: Article
• ISSN: 0022-3549
• DOI: 10.1002/jps.2600670440

✦ Synopsis

A simple chromatographic procedure was developed for the separation and determination of theophylline and hydroxyethyltheophylline. An aliquot of diluted syrup was pipetted into a chromatographic column containing a strong anion-exchange resin, which retains theophylline and allows the hydroxyethyl derivative to pass through it. Theophylline was subsequently eluted with 1 N HCl and determined by difference spectrophotometry at 284 nm; its derivative was determined at 272 nm.