Separation and quantitative determination of sesquiterpene lactones in Lindera aggregata (Wu-yao) by ultra-performance LC-MS/MS

✍ Scribed by Yongjiang Wu; Yunliang Zheng; Xuesong Liu; Zheng Han; Yiping Ren; Lishe Gan; Changxin Zhou; Lianjun Luan

• Book ID: 102444803
• Publisher: John Wiley and Sons
• Year: 2010
• Tongue: English
• Weight: 232 KB
• Volume: 33
• Category: Article
• ISSN: 1615-9306
• DOI: 10.1002/jssc.200900768

✦ Synopsis

Abstract

An ultra‐performance LC in combination with MS/MS method was established to evaluate the quality of Wu‐yao (the dried root of Lindera aggregata (Sims) Kosterm. (Lauraceae)) through simultaneous quantitation of five sesquiterpene lactones, linderagalactone D (1), linderagalactone C (2), hydroylindestenolide (3), neolinderalactone (4) and linderane (5). All compounds were separated on a Waters Acquity ultra‐performance LC HSS T3 column (2.1 mm×100 mm, 1.8 μm particle size) with linear gradient elution of acetonitrile and 0.1% formic acid. An ESI interface in the positive mode was employed prior to MS detection. All the compounds showed good linearity (R^2^≥0.9992). The recoveries, measured at three concentration levels, varied from 97.3 to 103.4% with RSDs <4.8%. The simple, rapid and reliable method was successfully applied to quantify the five components in 13 samples of Wu‐yao from different areas.