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Separation and purification of Escherichia coli-expressed human thymosin-α1 using affinity chromatography and high-performance liquid chromatography

✍ Scribed by Hong-Ying Zhang; Pei-Fu Chen; Jia-Ming Xu; Quan-Min Dai; Feng Xu; Qing-Wang Han; Jian-Jun Wang; Hei-Ying Jin


Book ID
116829280
Publisher
Elsevier Science
Year
2011
Tongue
English
Weight
403 KB
Volume
77
Category
Article
ISSN
1046-5928

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## All naturally occurring sphingomyelins have the D-erythro-(2S,3R ) configuration of the sphingoid base. We have developed a normal-phase HPLC method for the separation of this natural stereoisomer from the L- threo-sphingomyelin, which is the other stereoisomer commonly present in semisynthetic