Separation and properties of EA-rosette-forming lymphocytes in humans

✍ Scribed by M.H.J. Van Oers; W.P. Zeijlemaker; P. T. H. A. Schellekens

• Publisher: John Wiley and Sons
• Year: 1977
• Tongue: English
• Weight: 853 KB
• Volume: 7
• Category: Article
• ISSN: 0014-2980
• DOI: 10.1002/eji.1830070306

✦ Synopsis

Abstract

Human peripheral blood lymphocytes were separated into subpopulations enriched or depleted with respect to B lymphocytes (Ig‐bearing cells), T lymphocytes, (cells forming rosettes with sheep erythrocytes: E‐RFC) and Fc receptor‐bearing lymphocytes (EA‐RFC).

From the distributions and recoveries of the various cell types it could be concluded that there was very little overlap between Ig‐bearing lymphocytes and EA‐RFC. The latter cells partly belonged to “null” (non‐T, non‐B) cells; it was however demonstrated that 30 % of the EA‐RFC were T cells (E‐RFC).

The lytic capacity in antibody‐dependent lymphocytotoxicity (ADL) was shown to correspond with the proportions of EA‐RFC in the various fractions. Non‐T cells showed enhanced ADL activity when compared to the unseparated cells. Purified T cell populations also displayed ADL activity. Since the latter could not be due to contaminating non‐T cells, this activity was ascribed to Fc receptor‐bearing T lymphocytes.