Separation and identification of unsaturated fatty acid isomers in blood serum and therapeutic oil preparations in the form of their oxazoline derivatives by GC-MS
✍ Scribed by Liebich, Hartmut M. ;Schmieder, Norbert ;Wahl, Hans Günther ;Wöll, Josef
- Publisher
- John Wiley and Sons
- Year
- 1994
- Tongue
- English
- Weight
- 255 KB
- Volume
- 17
- Category
- Article
- ISSN
- 0935-6304
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✦ Synopsis
Abstract
Fatty acids from blood serum lipids and fish oil preparations are derivatized with 2‐amino‐2‐methyl‐1‐propanol to form 2‐substituted 4,4‐dimethyloxazolines. The derivatives are separated on a 25 m × 0.25 mm FFAP column and identified by mass spectrometry using electron impact ionization. The positions of the double bonds of mono‐ and polyunsaturated fatty acids can be easily and reliably recognized from a characteristic fragmentation feature of the oxazolines. Several unsaturated C16 to C22 fatty acid isomers are identified whose exact structure has not been derived from the commonly used methyl esters.