Separation and identification of organic acids in root exudates of Lupinus luteus by capillary zone electrophoresis

✍ Scribed by C. Barbas; J. A. Lucas García; F. J. Gutiérrez Mañero

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 76 KB
• Volume: 10
• Category: Article
• ISSN: 0958-0344
• DOI: 10.1002/(sici)1099-1565(199903/04)10:2<55::aid-pca437>3.0.co;2-i

✦ Synopsis

The separation and identification of a mixture of organic acids by capillary electrophoresis, with direct detection by UV absorption at a wavelength of 200 nm is reported. A solution containing the following organic acids was studied: oxalic, formic, fumaric, malic, succinic, citric, acetic and lactic acid. Separation was achieved using a fused-silica capillary filled with phosphate buffer (200 mM; pH 6) containing N-acetyl-N,N,N-trimethylammonium bromide (0.5 mM) as cationic surfactant, necessary to modify the electrosmotic flow. The composition of organic acids in exudates of Lupinus luteus was determined by this method.