Separation and identification of aliphatic amine derivatives with a new fluorescent reagent using high-performance liquid chromatography and thin-layer chromatography
✍ Scribed by P. Jandera; H. Pechová; D. Tocksteinová; J. Churáček; J. Královský
- Book ID
- 112719340
- Publisher
- Springer
- Year
- 1982
- Tongue
- English
- Weight
- 558 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0009-5893
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A simple and highly sensitive method based on the derivatization of aliphatic amines with 2- (2-phenyl-1H-phenanthro-[9,10-d]imidazole-1-yl)-acetic acid (PPIA) followed by high-performance liquid chromatography (HPLC) with fluorescence detection and on-line post-column mass spectrometric identificat
Three fluorescent derivatization reagents for compounds having hydroxyl and/or amino groups are described. 4-( 2-Phthalimidyl)benzoyl chloride, 3-( 2-phthalimidyl)benzoyl chloride and 3-( 2-phthalimidyl)4-methoxybenzoyl chloride, prepared from the corresponding phthalimidylbenzoic acid, were stable
Figure 1. Reaction of D/L-amino acids with R(À)-DBD-PyNCS.