Separation and Enzymatic Saccharification of Cellulose from Wakame (Undaria pinnatifida)
✍ Scribed by Takashi Korenaga; Shin-ichiro Fujii
- Publisher
- Elsevier Science
- Year
- 2000
- Tongue
- English
- Weight
- 147 KB
- Volume
- 13
- Category
- Article
- ISSN
- 0889-1575
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✦ Synopsis
Cellobiose and glucose were produced using an enzyme (cellulase from ¹richoderma viride) reaction from the crude "ber unit (CFU) separated from Wakame seaweed (;ndaria pinnati,da). The method for separation involved adding sodium carbonate (Na CO ) to desalted stalks of wet Wakame. The wet CFU was pulverized using a centrifugal grinder after lyophilization. The enzyme reaction used the CFU powder as a substrate. The maximum concentration of cellobiose and glucose reached 56$2.0 and 130$0.2 mg/g, respectively. The total cellulose included in this CFU was determined to be about 70%. The sugar recovery from cellulose in the CFU was 20%. Although glucose was detected when micro-crystalline cellulose was used as a substrate, cellobiose and glucose were detected when CFU powder was used as a substrate.