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Separation and Assay of Cholecalciferol in Vitamin A + D Ointment

โœ Scribed by Mahmood Tajbakhsh; Manijeh Karimi; Mohammad Javad Chaichi; Mohammad A. Khalilzadeh; Saeed Haghighi


Publisher
Springer Vienna
Year
2007
Tongue
English
Weight
67 KB
Volume
139
Category
Article
ISSN
0026-9247

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The fluorogenic dienophile 1,2,4-triazoline-3,5-dione with a highly fluorescent quinoxalinone group at the 4-position (DMEQ-TAD) was synthesized and exploited as a reagent to assay vitamin D metabolites. 25-Hydroxyvitamin D3, 1 alpha,25-dihydroxyvitamin D3, and 24(R),25-dihydroxyvitamin D3 reacted q