Poly(vinyl pyrrolidone) solution was used as a separation matrix in capillary electrophoresis for DNA sequencing. Four-label four-color detection was performed for base calling. Dye-labeled DNA showed large mobility shifts at normal conditions for DNA separation. Temporal correction of mobility shif
Separating DNA sequencing fragments without a sieving matrix
โ Scribed by Hongji Ren; Achim E. Karger; Frank Oaks; Steve Menchen; Gary W. Slater; Guy Drouin
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 222 KB
- Volume
- 20
- Category
- Article
- ISSN
- 0173-0835
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## Abstract We present a โproofโofโconceptโ study showing that a blend of thermoโresponsive and nonthermoโresponsive polymers can be used to create a DNA sieving matrix with a thermally tunable mesh size, or โdynamic porosityโ. Various blends of two wellโstudied sieving polymers for CE, including h
## Matrix conditioning for lengthened capillary DNA sequencing Capillary electrophoresis (CE) is currently the preferred format for both DNA sequencing and small DNA fragment analysis. The present study provides a simple revision of the procedure used for CE of DNA with a commercial DNA sequencing