Sensitivity of the ΦX174 am3 allele in relation to the endogenous Hprt gene for detecting mutation in transgenic mice
✍ Scribed by James B. Chen; S. Balachandra Dass; James G. Burkhart; Robert H. Heflich
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 159 KB
- Volume
- 32
- Category
- Article
- ISSN
- 0893-6692
No coin nor oath required. For personal study only.
✦ Synopsis
Transgenic mice have been developed containing kg of ENU, the Hprt assay detected an average 22multiple, chromosomally integrated copies of the fold increase over background, while the am3 MFs FX174 am3 allele that serve as reporters for in vivo averaged threefold above background. With the mutation at a single A:T basepair. In this study, we 160 mg/kg dose, the Hprt assay detected a 54-fold examined the relative sensitivity of the am3 average increase, while a sixfold average increase transgene for detecting the in vivo mutagenicity of above background was found for the transgenic N-ethyl-N-nitrosourea (ENU). Three-week-old male locus. We conclude that the sensitivity of the am3 FX174 mice were treated with 0, 40, and 160 assay to ENU was compromised by the presence mg/kg of ENU. After 1, 3, 6, and 9 weeks, animals of ex vivo mutations. Adjustment of am3 MFs to were killed, their spleens removed, and isolated exclude these ex vivo mutants could enhance the splenocytes were used to measure mutant frequen-sensitivity of the assay. Environ. Mol. Mutagen. cies (MFs) in both the am3 allele and the endoge-32: 229 -235, 1998 ᭧ 1998 Wiley-Liss, Inc. nous Hprt gene. For animals treated with 40 mg/