Sensitivity of Electrochemically Nanostructured Carbon Fiber Ultramicroelectrodes in the Determination of Adenosine

✍ Scribed by Kholoud Abou El-Nour; Anna Brajter-Toth

• Publisher: John Wiley and Sons
• Year: 2000
• Tongue: English
• Weight: 164 KB
• Volume: 12
• Category: Article
• ISSN: 1040-0397
• DOI: 10.1002/1521-4109(200007)12:11<805::aid-elan805>3.0.co;2-x

✦ Synopsis

A direct and fast voltammetric method of determination of adenosine, with a novel carbon ®ber ultramicroelectrode (CFE), is demonstrated here. To achieve high sensitivity required in biological determinations, before the determination of adenosine, carbon ®ber ultramicroelectrodes were activated by electrochemical pretreatment. The electrochemical activation process generates a nanostructured carbon ®ber surface which is stable. The high stability of the active surface, and the high reproducibility of the activation process, leads to reproducible and sensitive fast scan voltammetric (FSV) determinations of adenosine. Adenosine sensitivity was determined in 7610 72 M phosphate buffer, pH 7.4, at a scan rate of 500 V s 71 . The potential window in the determinations was from 7 1.0 to 1.5 V (vs. SCE). A high sensitivity of the new method of adenosine determinations was achieved under physiological conditions.