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SENSITIVITY OF DAUDI CELLS TO TUMOR NECROSIS FACTOR ALPHA: EARLY CHANGES IN POLYPHOSPHOINOSITIDE METABOLISM

✍ Scribed by E. SANTAVENERE; R. DI PIETRO; L. DI DOMENICANTONIO; R. RANA

Book ID
102565706
Publisher
Elsevier Science
Year
1996
Tongue
English
Weight
300 KB
Volume
20
Category
Article
ISSN
1065-6995

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✦ Synopsis

The effects of recombinant Tumor Necrosis Factor (r-TNF ) on polyphosphoinositide metabolism were examined in a Burkitt Lymphoma cell line (Daudi cells). After 1 h of in vitro treatment with r-TNF , the incorporation of 32 Pi into phosphatidylinositol 4,5-phosphate (PtdInsP 2 ), phosphatidylinositol 4-phosphate (PtdInsP) and phosphatidylinositol (PtdIns) was reduced compared with controls, confirming previous findings observed in other cell lines of a specific PtdIns breakdown following r-TNF treatment. The novelty of this study is therefore the demonstration of early changes in polyphosphoinositide metabolism during the antiproliferative response elicited by this cytokine in Daudi cells.

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