Sensitivity-Improved Detection of Protein Hydration and Its Extension to the Assignment of Fast-Exchanging Resonances

Evaluation of a dual-wavelength size exc

Evaluation of a dual-wavelength size exclusion HPLC method with improved sensitivity to detect protein aggregates and its use to better characterize degradation pathways of an IgG1 monoclonal antibody

✍ Michael D. Bond; Mark E. Panek; Zheru Zhang; Dana Wang; Promod Mehndiratta; Hui 📂 Article 📅 2010 🏛 John Wiley and Sons 🌐 English ⚖ 380 KB

The evaluation of a dual wavelength size exclusion high performance liquid chromatography (DW-SE-HPLC) method with improved sensitivity to detect aggregates in a high concentration IgG1 monoclonal antibody formulation is presented. This technique utilizes ultraviolet detection at two different wavel