𝔖 Bobbio Scriptorium
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Sensitivity Enhancement in the TROSY Experiment

✍ Scribed by M. Czisch; R. Boelens

Book ID
102596514
Publisher
Elsevier Science
Year
1998
Tongue
English
Weight
63 KB
Volume
134
Category
Article
ISSN
1090-7807

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✦ Synopsis

A modification of the TROSY experiment which allows for sensitivity enhancement without introduction of additional delays is presented. The method relies on the combination of two orthogonal pathways and results in a signal-to-noise gain of ͌ 2 independent of the size of the molecule. The sensitivity enhanced TROSY scheme can be combined with gradient coherence selection. The method has been applied to the 269 residue serine protease PB92.

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In (1)H-(15)N TROSY experiments of proteins and nucleic acids, where the second coherence transfer delay time tau' has been fixed as 5.6 ms, 1/(2(1)J(NH)), in order to achieve complete spin-state selection, spurious negative peaks are observed along the (15)N axes. These peaks are often annoyingly l