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Sensitivity Enhancement in Solid State 15N NMR by Indirect Detection with High-Speed Magic Angle Spinning

✍ Scribed by Yoshitaka Ishii; Robert Tycko

Publisher
Elsevier Science
Year
2000
Tongue
English
Weight
82 KB
Volume
142
Category
Article
ISSN
1090-7807

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✦ Synopsis

Enhancement of sensitivity in solid state 15 N NMR by indirect detection through 1 H NMR signals under high-speed magic angle spinning and high-field conditions is demonstrated experimentally on two 15 N-labeled peptides, polycrystalline AlaGlyGly and the helix-forming, 17-residue peptide MB(i ؉ 4)EK in lyophilized form. Sensitivity enhancement factors ranging from 2.0 to 3.2 are observed experimentally, depending on the 15 N and 1 H linewidths and polarization transfer efficiencies. The 1 H-detected two-dimensional 1 H/ 15 N correlation spectrum of AlaGlyGly illustrates the possibility of increased spectral resolution and resonance assignments in indirectly detected experiments, in addition to the sensitivity enhancement.

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