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Sensitive polarographic estimation of thiol groups with N-ethylmaleimide

✍ Scribed by P.D.J. Weitzman; Hilary J. Tyler

Book ID
102630016
Publisher
Elsevier Science
Year
1971
Tongue
English
Weight
173 KB
Volume
43
Category
Article
ISSN
0003-2697

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πŸ“œ SIMILAR VOLUMES

Assay of thiols and disulfides based on
Assay of thiols and disulfides based on the reversibility of N-ethylmaleimide alkylation of thiols combined with electrolysis
✍ Junko Nishiyama; Toyo Kuninori πŸ“‚ Article πŸ“… 1992 πŸ› Elsevier Science 🌐 English βš– 544 KB

A simple and specific method for analyzing thiols and disulfides on the basis of the reversibility of N-ethylmaleimide (NEM) alkylation of thiols is described. When the adduct of NEM and glutathione (GSH) was electrolyzed at neutral pH, all of the GSH was recovered. When the adduct was exposed to pH