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Sensitive Nonradioactive Detection of mRNA in Tissue Sections: Novel Application of the Whole-mount In Situ Hybridization Protocol

✍ Scribed by Moorman, A. F. M.; Houweling, A. C.; de Boer, P. A. J.; Christoffels, V. M.


Book ID
124159595
Publisher
Histochemical Society
Year
2001
Tongue
English
Weight
549 KB
Volume
49
Category
Article
ISSN
0022-1554

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The growth-associated protein GAP-43 is a presynaptic membrane phosphoprotein that is expressed at high levels during development and axonal growth. To evaluate the cellular distribution of GAP-43 mRNA in the hippocampus and cerebellum of adult rats we applied in situ hybridization (ISH) as well as