Sensitive high-pressure liquid chromatographic determination of disopyramide and mono-N-dealkyldisopyramide

✍ Scribed by Gloria Nygard; William H. Shelver; S. K. Wahba Khalil

• Book ID: 102404405
• Publisher: John Wiley and Sons
• Year: 1979
• Tongue: English
• Weight: 353 KB
• Volume: 68
• Category: Article
• ISSN: 0022-3549
• DOI: 10.1002/jps.2600681033

✦ Synopsis

A high-pressure liquid chromatographic procedure for the accurate determination of disopyramide and its chief metabolite in plasma is presented. The method is suitable for monitoring patients receiving disopyramide therapy. A reversed-phase cyanopropylsilane column is utilized with a mobile phase of 50% acetonitrile and 50% 0.01 M sodium acetate buffer at pH 4.0. Absorption was monitored at 254 nm with a detection limit of 0.2 microgram/ml of plasma. The reproducibility and precision of the procedure were demonstrated on samples containing 0.50-12 microgram/ml of plasma.