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Sensitive genotyping of Cryptosporidium parvum by PCR-RFLP analysis of the 70-kilodalton heat shock protein (HSP70) gene

✍ Scribed by Philippe Gobet; Simon Toze


Book ID
109324987
Publisher
John Wiley and Sons
Year
2001
Tongue
English
Weight
283 KB
Volume
200
Category
Article
ISSN
0378-1097

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Polymerase chain reaction (PCR)-coupled single-strand conformation polymorphism (SSCP) approaches utilizing nuclear DNA regions of the small subunit (SSU) of ribosomal RNA and heat shock protein 70 gene (HSP70) were established for genotyping Cryptosporidium parvum. The regions were amplified (indiv