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Sensitive fluorescence in situ hybridization signal detection in maize using directly labeled probes produced by high concentration DNA polymerase nick translation

✍ Scribed by Kato, A; Kato, A; Albert, PS; Vega, JM; Kato, A; Albert, PS; Vega, JM; Birchler, JA


Book ID
118043236
Publisher
Informa plc
Year
2006
Tongue
English
Weight
466 KB
Volume
81
Category
Article
ISSN
1052-0295

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This study was carried out to determine whether Y-bearing porcine spermatozoa could be detected by in situ hybridization using a digoxigenin (Dig)-labelled DNA probe specific to the Y chromosome produced by polymerase chain reaction (PCR). A conventional PCR (with Dig-dUTP) was performed using a set