Sensitive fluorescence assays for urokinase using synthetic peptide 4-methoxy-β-naphthylamide substrates
✍ Scribed by William L. Bigbee; Howard B. Weintraub; Ronald H. Jensen
- Publisher
- Elsevier Science
- Year
- 1978
- Tongue
- English
- Weight
- 492 KB
- Volume
- 88
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
✦ Synopsis
Two assays for the plasminogen activator urokinase using peptide fluorogenie substrates are described. N-carbobenzoxy-glycyl-glycyl-L-arginine-4-methoxy+-naphthylamide (CBZ-Gly-Gly-Arg-4MPNA) can be used in a direct assay that is simple, rapid, and sensitive to as little as 0.5 III/ml urokinase. Additional sensitivity, to 0.01 IUiml urokinase, is obtained in a second method that uses plamsinogen as the primary substrate followed by a fluorogenic substrate assay employing N-carbobenzoxy-L-alanyl-L-alanyl-L-lysine-4methoxy-/3-naphthylamide (CBZ-Ala-Ala-Lys-4MPNA) as a specific substrate for the activated plasmin. These assays are as sensitive as the best assays presently in use and are simpler to perform. In addition, these assays can readily be used for kinetic analysis of the hydrolytic activity of urokinase or other plasminogen activators.