𝔖 Bobbio Scriptorium
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Sensitive enzyme assays based on the production of chemiluminescent leaving groups

✍ Scribed by Bruce R. Branchini; Jeffrey D. Hermes; Francesco G. Salituro; Nancy J. Post; Göran Claeson

Book ID
102985679
Publisher
Elsevier Science
Year
1981
Tongue
English
Weight
805 KB
Volume
111
Category
Article
ISSN
0003-2697

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✦ Synopsis

A new type of synthetic peptide substrate for amidase assay has been devised. The substrates are luminogenic, with potential for extremely high sensitivity, and are here exemplified by Boc-and Z-Ala-Ala-Phe-isoluminol amide. The synthetic substrates were designed to release isoluminol when hydrolyzed by enzyme; isoluminol production was determined by measuring its chemiluminescence. Kinetic constants of the luminogenic substrates were measured with a-chymotrypsin; and levels of the enzyme as low as 50 ng were determined conveniently. A comparison of similar luminogenic, chromogenic, and fluorogenic substrates is presented.

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