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Sensitive determination for adenylate cyclase activity by cyclic adenosine 3′,5′-monophosphate protein binding assay

✍ Scribed by U. Schwabe; R. Ebert; P. S. Schönhöfer


Book ID
112342532
Publisher
Springer-Verlag
Year
1974
Tongue
English
Weight
804 KB
Volume
286
Category
Article
ISSN
0028-1298

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Assay of Adenosine 3′,5′-Cyclic Monophos
✍ R.P. Newton; A.M. Evans; J.I. Langridge; T.J. Walton; F.M. Harris; A.G. Brenton 📂 Article 📅 1995 🏛 Elsevier Science 🌐 English ⚖ 638 KB

Cyclic AMP-dependent protein kinase is conventionally assayed by measuring the incorporation of radiolabeled phosphate into a histone substrate. Here the assay of the protein kinase is carried out by the positive-ion fast atom bombardment mass spectrometric analysis of the enzyme incubation mixture