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Sensitive Detection of Polyalanine Expansions in PHOX2B by Polymerase Chain Reaction Using Bisulfite-Converted DNA

✍ Scribed by Horiuchi, Hidekazu; Sasaki, Ayako; Osawa, Motoki; Kijima, Kazuki; Ino, Yukiko; Matoba, Ryoji; Hayasaka, Kiyoshi


Book ID
123220305
Publisher
American Society for Investigative Pathology
Year
2005
Tongue
English
Weight
181 KB
Volume
7
Category
Article
ISSN
1525-1578

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Detection of Y-bearing porcine spermatoz
✍ Tatsuo Kawarasaki; Tetsuya Kohsaka; Masaru Sone; Mitsutoshi Yoshida; Kimio Bamba πŸ“‚ Article πŸ“… 1995 πŸ› John Wiley and Sons 🌐 English βš– 492 KB

This study was carried out to determine whether Y-bearing porcine spermatozoa could be detected by in situ hybridization using a digoxigenin (Dig)-labelled DNA probe specific to the Y chromosome produced by polymerase chain reaction (PCR). A conventional PCR (with Dig-dUTP) was performed using a set