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Sensitive detection of pesticides using a differential ISFET-based system with immobilized cholinesterases

✍ Scribed by A.M.Nyamsi Hendji; N. Jaffrezic-Renault; C. Martelet; P. Clechet; A.A. Shlu'ga; V.I. Strikha; L.I. Netchiporuk; A.P. Soldatkin; W.B. Wlodarski

Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
674 KB
Volume
281
Category
Article
ISSN
0003-2670

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✦ Synopsis

Orsanophospho~~ pesticides were determined using a simple biosensor design. Enzymatic membranes prepared by cross-linking with BSA were deposited on a sensor chip consisting of two identical pH-FETs working in the differential mode (BNFET with enzyme, REFET without enzyme). Acetyl-and butyryicholinesterase membranes were tested using acetyl-and butyrylcholine chloride as substrates. The decrease in the substrate steady-state response caused by exposure of the biosensor to pesticides [diisopropyl fluorophosphate (DFP), trichlorfon, paraoxon-methyl] was used to estimate the enxyme inhibition. The optimized parameters for the measurement procedure were determined and the detection limits were found to be 10-l' M for DFP and 10m6 M for paraoxon-methyl and trichlorfon. k?yword~ Biosensors; Enzymatic methods; Cholinesterases; Ion-sensitive field effect transistors; Organophosphorus pesticides; Pesticides

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