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Sensitive Detection of Near-Infrared Fluorescent Dyes Using High-Performance Liquid Chromatography with Peroxyoxalate Chemiluminescence Detection System

✍ Scribed by Kohsuke Kimoto; Ryoya Gohda; Katsuhisa Murayama; Tomofumi Santa; Takeshi Fukushima; Hiroshi Homma; Kazuhiro Imai

Publisher: John Wiley and Sons
Year: 1996
Tongue: English
Weight: 161 KB
Volume: 10
Category: Article
ISSN: 0269-3879
DOI: 10.1002/(sici)1099-0801(199607)10:4<189::aid-bmc585>3.0.co;2-p

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✦ Synopsis

The highly sensitive detection of four near-infrared (near-IR) fluorescent dyes, methylene blue, pyridine 1, oxazine 1 and 3,3'-diethylthiadicarbocyanine iodide (DTDCI) by high-performance liquid chromatography (HPLC) with post-column peroxyoxalate chemiluminescence (PO-CL) detection utilizing bis(4-nitro-2-(3,6,9-trioxadecyloxycarbonyl)phenyl) oxalate (TDPO) and hydrogen peroxide was examined. The detection limits for methylene blue, pyridine 1, oxazine 1 and DTDCI were 120,27,31,0.19 fmol on-column at a signal-tonoise ratio of 2, respectively. The sensitivity for DTDCI was 250 times that obtained by HPLC with the conventional fluorescent detection.

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