Sensitive bioluminescent detection of pesticides utilizing a membrane mutant of Escherichia coli and recombinant DNA technology

✍ Scribed by Soomi Lee; Masayasu Suzuki; Eiichi Tamiya; Isao Karube

• Publisher: Elsevier Science
• Year: 1992
• Tongue: English
• Weight: 512 KB
• Volume: 257
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(92)85169-7

✦ Synopsis

The development of a mlcrobnxl sensmg system utlhzmg a membrane mutant of Eschenchra co& and recombmant DNA technology for the determmatlon of pesucldes m the enwronment IS described The enusslon of hght m E cob was achieved by cloning the genes encodmg firefly h&erase, and by Ityectmg the luminescent substrate, luctierm A consrderable Increase m sensltlvlty to toxx. substances was obtamed unng the membrane mutant of E cob as compared with the wdd type Pestuxdes and herbxxies could be detected by the decrease of 111 wo lummescence at concentrations down to 50 pg 1-l