Sensitive 1H-31P correlations with 5′ methylene protons of DNA via homonuclear double-quantum coherence

C5′ Methylene Proton Signal Assignment o

C5′ Methylene Proton Signal Assignment of DNA/RNA Oligomers Labeled with C5′-Monodeuterated Nucleosides by 1H–31P HSQC Spectroscopy

✍ Akira Ono; Takako Makita; Shin-ichi Tate; Etsuko Kawashima; Yoshiharu Ishido; Ma 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 582 KB

A new strategy for assigning the methylene protons attached to the C5' of nucleic acids is described for a DNA dodecamer duplex, d(CGCGAATTCGCG),. It employs 2D 'H-j'P HSQC spectroscopy for DNA composed of deoxyribonucleotides, having one deuteron at their C5' positions. Due to the lack of scalar as