Semisynthesis and properties of a fluorescent phosphatidyl-inositol analogue containing a cis-parinaroyl moiety

✍ Scribed by Pentti Somerharju; Karel W.A. Wirtz

• Publisher: Elsevier Science
• Year: 1982
• Tongue: English
• Weight: 575 KB
• Volume: 30
• Category: Article
• ISSN: 0009-3084
• DOI: 10.1016/0009-3084(82)90009-3

✦ Synopsis

A method for the preparation of a fluorescent phosphatidylinositol analogue, l-acyl,2parinaroyl-sn-glycero-3-phospho-sn-l-myo-inositol has been developed. This method makes use of yeast phosphatidylinositol as the starting material and includes the following steps: (1) acetylation of the free hydroxyl groups of the inositol moiety; (2) removal of the fatty acid from the sn-2-position of the glycerol moiety by phospholipase A2; (3) reacylation with parinaroyl anhydride; (4) removal of the protecting acetyl groups by alkaline solvolysis. The identity of the product was established by thin-layer chromatography (TLC), UV absorption spectroscopy, enzymatic degradation and by a transfer assay using the phosphatidylinositol transfer protein from bovine brain. Some properties of the fluorescent phosphatidylinositol analogue are reported.