Semiquantitative analysis of urinary low protein levels using silver dot blot assay
✍ Scribed by Kazuyuki Matsuda; Nobuo Hiratsuka; Yuriko Kurihara; Kiyoko Shiba
- Publisher
- John Wiley and Sons
- Year
- 2001
- Tongue
- English
- Weight
- 126 KB
- Volume
- 15
- Category
- Article
- ISSN
- 0887-8013
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✦ Synopsis
Abstract
We designed a semiquantitative analysis of urinary low protein levels using silver dot blot assay. In this method, 3 μl of urine are blotted to one dot onto a cellulose acetate membrane, which is stained by a colloidal silver staining reagent, and the optical density of the silver stained urinary protein is measured at 500 nm using a densitometer. There was a good linearity between 2.5 mg/L and 100 mg/L and a gentle linearity between 100 mg/L and 200 mg/L, and the minimum sensitivity was 2.5 mg/L. This method is suitable for semiquantitative analysis of urinary protein levels less than 300 mg/L, which can not be determined precisely by dipstick. J. Clin. Lab. Anal. 15:171–174, 2001. © 2001 Wiley‐Liss, Inc.