Semiautomated gel chromatography to characterize broad molecular weight distributions of cytoplasmic extracts from coastal bermuda grass
✍ Scribed by M.L. Fishman
- Book ID
- 102983858
- Publisher
- Elsevier Science
- Year
- 1976
- Tongue
- English
- Weight
- 563 KB
- Volume
- 74
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
✦ Synopsis
A stable semiautomated gel chromatograph was developed and can fractionate globular molecules whose sizes range from 340 to 1,000,000 daltons. The instrument was used to characterize size distributions of molecules in the cytoplasmic fraction of coastal bermuda grass (Cynodon dactylon (L.) Pers.). Distribution was continuous over the entire fractionating range of the gel. Further, the apparent molecular weight distribution depended upon the method of detection. Thus, the apparent first moment molecular weights were 5180, 10,800, and 32,000 daltons, as determined, respectively, by refractive index measurements and by absorbance readings at 254 and 206 nm.