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Semi-quantitative detection of cytomegalovirus DNA from native serum and plasma by nested PCR: influence of DNA extraction procedures

✍ Scribed by Klaus Hamprecht; Elfriede Mikeler; Gerhard Jahn

Book ID: 114140458
Publisher: Elsevier Science
Year: 1997
Tongue: English
Weight: 197 KB
Volume: 69
Category: Article
ISSN: 0166-0934
DOI: 10.1016/s0166-0934(97)00148-1

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## Abstract A novel nested quantitative‐competitive polymerase chain reaction (nQC‐PCR) assay was developed to quantify as few as ten copies per tube of human cytomegalovirus DNA with an overall dynamic range of 10–10^5^ copies per tube. This nQC‐PCR assay is based on co‐amplification of a mimic DN