✦ LIBER ✦

Semi-micro methods for analysis of labile sulfide and of labile sulfide plus sulfane sulfur in unusually stable iron-sulfur proteins

✍ Scribed by Helmut Beinert

Publisher: Elsevier Science
Year: 1983
Tongue: English
Weight: 439 KB
Volume: 131
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(83)90186-0

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✦ Synopsis

Details are provided for a reproducible procedure for determination of labile sulfide in ironsulfur (Fe-S) proteins in the range of 1 to 3 nmol. Analyses are also presented on the most stable Fe-S protein so far reported. In this case denaturation with guanidine . HCl was used in the presence of dithiothreitol. The values obtained then also include any sulfane sulfur (So) present.