Semi-continuous operation of the insect cell — Baculovirus system
✍ Scribed by P. R. Piergiovanni; K. Dutt
- Book ID
- 104644723
- Publisher
- Springer-Verlag
- Year
- 1993
- Tongue
- English
- Weight
- 318 KB
- Volume
- 7
- Category
- Article
- ISSN
- 0951-208X
No coin nor oath required. For personal study only.
✦ Synopsis
The insect cell -baculovirus system is used to synthesize a variety of recombinant proteins, generally in a batch system. We have developed a semi-continuous system and studied its operation at three different residence times. The resulting data showed that beta-galactosidase can be produced for 50 to 150 hours longer than the batch system.
📜 SIMILAR VOLUMES
The inability to produce eukaryotic glycoproteins with complex N-linked glycans is a major limitation of the baculovirus-insect cell expression system. Recent studies have demonstrated that metabolic engineering can be used to extend the glycoprotein processing capabilities of lepidopteran insect ce