𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Semi-continuous enzymatic synthesis of cefaclor enhanced by in situ product removal

✍ Scribed by Liu Yang; Dongzhi Wei; Yewang Zhang

Publisher
Wiley (John Wiley & Sons)
Year
2004
Tongue
English
Weight
159 KB
Volume
79
Category
Article
ISSN
0268-2575

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Enzymatic synthesis of cefaclor was carried out with kinetic control. The product yield was improved by the continuous removal of product from the reaction mixture via complexation of cefaclor with 1‐naphthol. The effects of pH and temperature on the enzymatic and complexing reactions were investigated. The efficiency of the enzymatic conversion of cefaclor in the complexing reaction was 80% under optimum reaction conditions. In situ product removal (ISPR) decreased product concentration in the bioreactor, consequently the yield of cefaclor increased from 57% (without ISPR) to 80% (with ISPR). The specially designed reactor allowed enzymatic reaction and product removal to be accomplished simultaneously, in which the productivity of cefaclor was improved to 65 g dm^−3^ by semi‐continuous operation lasting for 55 h. Copyright © 2004 Society of Chemical Industry

📜 SIMILAR VOLUMES

In-situ product removal to enhance the y
In-situ product removal to enhance the yield of biocatalytic reactions with competing equilibria: α-glucosidase catalysed synthesis of disaccharides
✍ Farjad Ahmed; Andreas Stein; Gary J Lye 📂 Article 📅 2001 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 139 KB

## Abstract Transglycosylations are an important class of enzyme‐catalysed reaction that occur in most living organisms and which are finding increasing application for the synthesis of therapeutic compounds. Compared with other bioconversion processes, however, they generally suffer from low produ