Semi-Constant-Time HMSQC (SCT-HMSQC-HA) for the Measurement of 3JHNHα Couplings in 15N-Labeled Proteins
✍ Scribed by Helena Aitio; Perttu Permi
- Publisher
- Elsevier Science
- Year
- 2000
- Tongue
- English
- Weight
- 109 KB
- Volume
- 143
- Category
- Article
- ISSN
- 1090-7807
No coin nor oath required. For personal study only.
✦ Synopsis
A simple method for accurately measuring (3)J(H(N))(H(alpha)) coupling constants in (15)N-labeled proteins is described. This semi-constant-time HMSQC-HA experiment combines the rapidity and convenience of the recently introduced CT-HMQC-HA scheme (Postingl and Otting, J. Biomol. NMR 12, 319-324 (1998)) with the high resolution and robustness of the HSQC experiment. The proposed method is demonstrated for the 76-residue human ubiquitin and Saccharopolyspora erythraea calerythrin (176 residues). Our results imply that the SCT-HMSQC-HA experiment is suitable also for proteins with less favorable NMR properties due to its good resolution and sensitivity.