Semi-Automated fluorometric assay for urinary total and b n-acetyl-β-d-glucosaminidase: Analytical investigation
✍ Scribed by William Kristz; Ghassan Joseph Samaan; Corinne Leberre; Jean-François Demelier; Daniel Biou
- Publisher
- John Wiley and Sons
- Year
- 1991
- Tongue
- English
- Weight
- 188 KB
- Volume
- 5
- Category
- Article
- ISSN
- 0887-8013
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✦ Synopsis
Total N-acetyl-p-D-glucosaminidase (NAG) and NAG-B isoenzyme determination are determined in order to investigate renal tubular function. Here, we propose a semiautomated adaptation of Sandman's manual method for the Monarch centrifugal analyzer. Fluorescence of the released 4-methylumbelliferone is automatically read and results are directly expressed in nanokatalA. Mean (1 SD) values expressed in nanokatal/millirnole creatinine in urine from healthy female (n = 30) and male (n = 30) subjects were 8.2 (4.0) and 7.8 (2.9) for total NAG and 1.9 (1.4) and 1.5 (0.7) for the NAG-B isoenzyme activity. The assay of six patients with various renal disorders shows definite increase in total NAG and NAG-B isoenzyme activities.