Semi-automated assay of erythrocyte Cu-Zn superoxide dismutase activity

✍ Scribed by J.C. Fulbert; M. Succari; M.J. Cals

• Publisher: Elsevier Science
• Year: 1992
• Tongue: English
• Weight: 394 KB
• Volume: 25
• Category: Article
• ISSN: 0009-9120
• DOI: 10.1016/0009-9120(92)80054-k

✦ Synopsis

A semi-automated enzymatic colorimetric method for determining Cu-Zn superoxide dismutase (SOD) in erythrocytes using an FP9 parallel analyzer is described. The method is based on the reaction described by McCord and Fridovich (xanthine/ xanthine oxidase, ferricytochrome C), which permits kinetic measurements to be determined colorimetrically for the assay of enzyme activity. The coefficients of variation for within-and between-run analyses were <6%. The limit of linearity is 3.5-times the mean value of the normal range while recovery of added SOD ranged from 91 to 120%. The method, which is both reliable and simple, allows rapid, simultaneous measurements of multiple samples.

In the technique of L' Abb~ and Fischer (11), which is based on the reaction described by McCord and Fridovich (12), the xanthine/xanthine oxidase couple is associated with cytochrome C reduction. In this paper we describe a method for assaying erythrocyte SOD (E-SOD) based on the same principle, and we include the results of a study performed after adapting the technique to a parallel analyzer. We also report the values of E-SOD observed using the proposed method in healthy adults of both sexes.