Self-renewal of B-1 lymphocytes is dependent on CD19

✍ Scribed by Ian Krop; Antonin R. De Fougerolles; Richard R. Hardy; Michael Allison; Mark S. Schlissel; Douglas T. Fearon

• Book ID: 102823912
• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 526 KB
• Volume: 26
• Category: Article
• ISSN: 0014-2980
• DOI: 10.1002/eji.1830260137

✦ Synopsis

Self-renewal of B-1 lymphocytes is dependent on CD19

The B-l subset of B lymphocytes is maintained by self-renewal of mature cells, and this process may involve signaling through membrane immunoglobulin (mIg). We determined whether CD19, a membrane protein that co-stimulates B cells by mIg, has a role in this process. Pre-natal treatment of mice with 1D3, a rat anti-mouse CD19 monoclonal antibody, down-regulated CD19 expression and reduced by sixfold the number of B-la cells at birth; B-2 cells were relatively unaffected. Prolonged treatment of adult mice with ID3 caused the loss of approximately 2 % per day of peritoneal B-la cells, without diminishing the recovery of splenic B-2 cells. The loss of B-la cells was associated with inhibition of their replication rather than with accelerated turnover. Therefore, CD19 is involved in the development and self-renewal of B-la cells, perhaps through its ability to amplify signaling through mIgM.