✦ LIBER ✦
Self-Quenched Covalent Fluorescent Dye–Nucleic Acid Conjugates as Polymeric Substrates for Enzymatic Nuclease Assays
✍ Scribed by Vladimir S. Trubetskoy; James E. Hagstrom; Vladimir G. Budker
- Publisher
- Elsevier Science
- Year
- 2002
- Tongue
- English
- Weight
- 65 KB
- Volume
- 300
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
A fluorescent method is described for assessing nuclease activity. The technique is based on the preparation of quenched fluorophore-nucleic acid covalent conjugates and their subsequent dequenching due to degradation by nucleases. The resulting fluorescence increase can be measured by a spectrofluorometer and exhibits subpicogram per milliliter sensitivity level for RNase A and low picogram per milliliter level for DNase I. The method is adaptable for quantitative nuclease inhibitor testing.