Self-Assembly of Crosslinked DNA–Gold Nanoparticle Layers Visualized by In-Situ Scanning Force Microscopy
✍ Scribed by B. Zou; B. Ceyhan; U. Simon; C. M. Niemeyer
- Publisher
- John Wiley and Sons
- Year
- 2005
- Tongue
- English
- Weight
- 335 KB
- Volume
- 17
- Category
- Article
- ISSN
- 0935-9648
No coin nor oath required. For personal study only.
✦ Synopsis
Nanoparticle Preparation and Composition: See Supporting Information for details.
Microconstruct Preparation: A 0.05 mL droplet of the water±nanoparticle dispersion (concentration: 1 g L ±1 ) of poly(St-co-AN-co-PEGm)s was placed on a glass plate (washed by piranha treatment), which was set on the stage of the scanning electron microscope by using double-sided carbon adhesive tape (Fig. 1b). The droplet was not in direct contact with the liquid nitrogen, but the stage was partly immersed into it, and soon the droplet was frozen, presumably from the bottom up. It took less than 0.1 s for the droplet to freeze completely. Just after freezing, the samples were dried in vacuo (1.5 Pa) for 12 h to yield a white powder. Microconstruct formation was confirmed by SEM (HITACHI S-4100H SEM, 3 kV, 10 lA) observations, for which the specimen was coated by OsO 4 of 5 nm thickness.