Self- and Cross-Fertilization in the Solitary Ascidian Ciona savignyi
β Scribed by Di Jiang and William C. Smith
- Book ID
- 124906332
- Publisher
- Marine Biological Laboratory
- Year
- 2005
- Tongue
- English
- Weight
- 264 KB
- Volume
- 209
- Category
- Article
- ISSN
- 0006-3185
- DOI
- 10.2307/3593128
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π SIMILAR VOLUMES
The solitary ascidians Ciona intestinalis and Ciona savignyi co-occur in southern California harbors, but no hybrids have been recognized in nature. Numerous differences in their egg morphology were detected. Homologous (normal outcross) fertilization yielded 96-99% cleavage, where autologous (self)
## Abstract Summary: We report generation of stable transgenic lines of the ascidian __Ciona savignyi__ carrying a __Ciona intestinalisβBrachyury__βpromoter/__Green Fluorescent Protein__βreporter (__CiβBraβGFP__) construct. The transgenic lines were made using a technique in which the endonuclease