Self-absorption and re-emission in wavelength-dependent fluorescence decay
✍ Scribed by Jürg Baumann; Gion Calzaferri; Thomas Hugentobler
- Publisher
- Elsevier Science
- Year
- 1985
- Tongue
- English
- Weight
- 465 KB
- Volume
- 116
- Category
- Article
- ISSN
- 0009-2614
No coin nor oath required. For personal study only.
✦ Synopsis
The ~nlluence of fluorescence self-absorplmn and re-ermss~on on th P detemunatlon of the decay parameters of the excited state 1s relnvestlgated The ,th re-emlsslon step IS treared as the convoluuon of step J -1 wth rhe system's Impulse response For a smgk-exponential decay funcnon, whose hfetlme may depend on the enusslon wavelength. we show on what condltlons the fluorescence re-emlsslon alters the absolute value of the hfellme but not 11s wavelength dependence The treatment can cas~iy be extended to multlple exponenuals An exphclt correction formula IS Bven for phase fluonmetry expenments Expenmentally observed dual-exponential. wavelength-dependent. fluorescence decay of rhodamme 6G at different concenlratlons m ethanol IS used to Illustrate the theoretIca results