Selenium binding proteins in tissues of normal and selenium responsive myopathic lambs

✍ Scribed by N.D. Pedersen; P.D. Whanger; P.H. Weswig; O.H. Muth

• Publisher: Elsevier Science
• Year: 1972
• Weight: 758 KB
• Volume: 2
• Category: Article
• ISSN: 0006-3061
• DOI: 10.1016/s0006-3061(00)80142-8

✦ Synopsis

Normal and selenium-responsive myopathic (white muscle disease) lambs were injected with selenium-75 either as sodium seIenite or seknomethionine. Selected tissues were removed at autopsy 16 hr after injection. The soluble iraction from homogenat= of these tissues was subjected to gel chromatography and the effluent monitored for radioactivity and protein content. The elution curves xere similar for both sodium selenite and seknomethionine injections. Selenium was bound to two Merent molecular weight proteins in the kidney and three different molecular weight proteins in the liver, pancreas, and plasma. Four different moleeuku weight seknium-binding proteins were found in heart and semitendinosus muscle of 2-week-old normal lambs, but only three were found in these tissues of myopathic lambs. This fourth protein, which u-as absent in myopathic iambs, corresponded to a molecular weight of approximately 10,000. Hou-ever, by six weeks of age rudimentary amounts of this protein were present in the tissues of the myopathic Iamb. This protein may be implicated in the prevention of white muscle disease in lambs. JBTRQDUCTION Over a decade ago Schwarz and Felts [1] demonstrated that selenium would protect, against liver necrosis in rats. Subsequent work has revealed that dietary selenium protects against white muscle disease in Iambs and calves [z, 71, exudative diathesis in chicks 131, myocardial disorders of poults [4], and skin and eye lesions of rats [5]_ The diversity of selenium deficiency signs In these animaIs suggests a complex, if not multiplicity,