Selectivity control in the anion-exchange chromatographic determination of saccharides in dairy products using pulsed amperometric detection

✍ Scribed by Jan van Riel; Cees Olieman

• Publisher: Elsevier Science
• Year: 1991
• Tongue: English
• Weight: 506 KB
• Volume: 215
• Category: Article
• ISSN: 0008-6215
• DOI: 10.1016/0008-6215(91)84005-y

✦ Synopsis

The separation of D-glucose, D-galactose. o-fructose, lactose, lactulose, saccharose, maltose, and malto-oligomers on an anion-exchange column in combination with pulsed amperometric detection is described.

Resolution was optimized by using separate sodium hydroxide and sodium acetate gradients, generated by a quaternary solvent delivery system. The influence of lithium, potassium.

or tetrabutylammonium ions on the separation was found to be negligible. The sample treatment of dairy products consisted of IOO-fold dilution with water, followed by ultrafiltration.

The recovery of the saccharides in milk was found to be 98.2 to 101.8%, while the repeatability was 0.7% to 5.6%. Determination of saccharides in an infant formula, in a fruit yogurt, and in a candy product are shown. The l.c. anion-exchange chromatographic system described enables the determination of the majority ofsaccharides present in food products in a single analysis.