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Selectively13C-enriched DNA:13C and1H assignments of a triple helix by two-dimensional relayed HMQC experiments

✍ Scribed by Olivier Bornet; Gérard Lancelot; Luc Chanteloup; Nguyen T. Thuong; Jean-Marie Beau

Book ID: 104647199
Publisher: Springer Netherlands
Year: 1994
Tongue: English
Weight: 402 KB
Volume: 4
Category: Article
ISSN: 0925-2738
DOI: 10.1007/bf00156621

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✦ Synopsis

We present NMR studies of an intramolecular triple helix, the three strands of which have been linked by a hexaethylene glycol chain. To overcome the generally encountered difficulties of assignment in the homopyrimidine strands, the carbon CI' of the pyrimidines were selectively 13C-enriched. Assignments of the aromatic and sugar protons were obtained from NOESY-HMQC and TOCSY-HMQC spectra. We show that the recognition of a DNA duplex by a third strand via triplex formation is easily carried out in solution by observing the changes of the 1H1'-13C1' connectivities as a function ofpH. Furthermore, the conformation of the sugars has been found to be C2'-endo, on the basis of the coupling constant values directly measured in an HSQC spectrum.

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