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Selective Translation of Leaderless mRNAs by Specialized Ribosomes Generated by MazF in Escherichia coli

✍ Scribed by Oliver Vesper; Shahar Amitai; Maria Belitsky; Konstantin Byrgazov; Anna Chao Kaberdina; Hanna Engelberg-Kulka; Isabella Moll


Book ID
116354654
Publisher
Elsevier Science
Year
2011
Tongue
English
Weight
1011 KB
Volume
147
Category
Article
ISSN
0092-8674

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Two spontaneous mutants of Escherichia coli strain KMBL-146 selected for resistance to the aminoglycoside antibiotic neamine show severe restriction of amber suppressors in vivo. Purified ribosomes from the mutant strains exhibit low neamine-induced misreading in vitro and a decreased affinity for t

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Protein S5 and S12 were isolated from 30S ribosomal subunits of two E. coli mutants highly resistant to the antibiotic neamine, and of the parental strain. Proteinchemical analyses on these proteins led to the following results: a) In protein S5 the arginine residue in peptide T2 of the parental str

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The effect on translational fidelity of a particular mutation in the gene coding for protein S5(rpxE) has been investigated. This mutation has the opposite effect of a restrictive strA mutation; in vivo, it relieves the restriction imposed by strA on the suppression of T4 nonsense mutants and result